Xenbase Image ID: 159598

Fig. 1. Gene walking and primer extension results for RCO. (A) Genomic DNA templates prepared using EcoRV (E) MscI (M), or ScaI (S) restriction enzymes were used for 1st and 2nd round PCR reactions (1, 2). Products were analyzed on a 1.5% agarose gel. Control 1st round reactions (C) contained no template, and 2nd round reactions used first round products as templates. Second round products (arrowheads) were obtained using EcoRV and MscI templates, but not in control reactions. Similar results were obtained for Prph2. (B) The transcription initiation site of the RCO gene was mapped by primer extension using total RNA as a template. A specific product of primer extension was seen in X. laevis retina RNA (R) using primer Xrcogsp2. No specific products were obtained from heart RNA (H) or a control reaction containing no RNA (N). DNA sequencing reactions using Xrcogsp2 are shown on the left (GATC). Similar analysis of X. laevisPrph2 failed to give any detectable products. Image published in: Moritz OL et al. (2002) Copyright © 2002. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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