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Fig. 2. Summary of PCR results. The relative sizes and positions of clones obtained by PCR gene walking are summarized above. Gene walking allowed us to obtain 2146 bp of novel sequence from Prph2 and 813 bp of novel sequence from RCO using genomic DNA templates prepared with MscI, EcoRV, and HincII restriction enzymes. Clones obtained by this method terminated with restriction enzyme half-sites, as indicated. Clones tested for promoter activity in transgenic X. laevis are indicated, as is the relative position of the large Prph2 fragment obtained by conventional PCR. Image published in: Moritz OL et al. (2002) Copyright © 2002. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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