Xenbase Image ID: 159600

Fig. 3. Promoter alignments. Sequences derived from X. laevisPrph2 and RCO genes aligned with previously cloned homologues. (A) The X. laevisPrph2 product obtained with MscI (see Fig. 2) aligned with human and mouse homologues. (B) The X. laevis RCO product obtained with EcoRV (Figs. 1 and 2) aligned with the A. carolinensis cone opsin promoter (Kawamura and Yokoyama, 1993). Completely conserved bases are highlighted green, identical bases are yellow, and similar bases are blue. Italics indicate regions of overlap with previously cloned cDNAs, used to confirm the specificity of the PCR. Interesting features of the sequences are noted. In the Prph2 alignment, the transcription initiation site of mouse Prph2 (Ma et al., 1995) is indicated by an arrow and numbered +1. A consensus Inr element is found within a conserved region (extended by blue dashed line) previously suggested to function as a TATAA element. A non-conserved TATAA element is also present upstream. For RCO, the transcription start site of the X. laevis gene is indicated by the arrow and numbered +1 (see Fig. 1). Conserved potential binding sites for the retina-specific transcription factors Rx (PCE1 sites) and Crx (OTX sites) were identified, as well as a conserved TATAA box and a glass-like element. The AT-rich sequence indicated by the dashed line immediately follows a conserved PCE1 site, and could be functionally similar to a BAT-1 site. The region that resembles a previously described X. laevis dispersed repeat sequence (Riggs and Taylor, 1987) is underlined. Image published in: Moritz OL et al. (2002) Copyright © 2002. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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