XB-IMG-159601
Xenbase Image ID: 159601
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Fig. 4. PCR products function as promoters in transgenic X. laevis. (A) Ventral view of a transgenic tadpole expressing GFP under control of RCO-0.9 (see Fig. 2). A fluorescence image (green) is superimposed on a bright field image (grayscale). Expression of GFP was seen in the eyes (e). No fluorescence was seen in the heart (arrow). The gut (g) is auto-fluorescent. (B) Similar ventral view of a tadpole expressing GFP under control of Prph2-1.2. Fluorescence was seen in the eyes (e) and the heart (arrow). (C) Dorsal view of a tadpole expressing GFP under control of RCO-0.9, with strong pineal expression (arrow) (eye fluorescence cannot be seen in this view.) (D) Three-quarters view of a tadpole expressing GFP under control of Prph2-1.2. The image is a grayscale representation of green fluorescence without superimposed bright field. Low-level GFP expression was observed in the muscles of the jaw (j) and heart (h). In panels A–D the anterior end of each tadpole is on the right side of the image, marked (a). (E) Section of a tadpole eye expressing GFP under control of Prph2-1.2. Within the retina, GFP expression was seen in the photoreceptor or outer nuclear layer (onl), but not the inner nuclear layer (inl) or ganglion cell layer (gcl). (red=wheat germ agglutinin, blue=Hoechst 33342 nuclear stain). (F) Similar results were obtained with RCO-0.9, although low-level expression was occasionally observed in lens. At higher magnification, we observed Prph2-1.2-driven expression of GFP in photoreceptors of all rod and cone types (G), while RCO-0.9 drove expression only in cones (H) (red=anti-rhodopsin, which labels rods but not cones). (I) Cones expressing GFP under control of RCO-0.9 were labeled by antibody cos-1 (red), which reacts with the X. laevis long-wavelength cone pigment (Rohlich et al., 1989). (J) In some transgenic retinas RCO-0.9-driven expression varied dramatically among cells, allowing individual cones to be imaged. As previously shown for rods (Moritz et al., 1999) GFP was distributed throughout the cones, but was found at lower concentrations in outer segments (os) and the mitochondria-containing region (m) and was excluded from oil droplets (od). Inner segment=is, synapse=s. A–D bar=100 μm, G–I bar=30 μm, J Bar=10 μm. Image published in: Moritz OL et al. (2002) Copyright © 2002. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |