Xenbase Image ID: 159602

Fig. 5. Endogenous Xrds38 mRNA is not detectable in heart tissue. (A) RT-PCR analysis of retina and heart RNA using primers designed to amplify a 956 bp fragment of the Xrds38 cDNA. RT-PCR was carried out as described in Section 2 using oligo-dT primed cDNA templates, and analyzed on a 1.5% agarose gel. cDNA templates were generated using total RNA from X. laevis retina (R), heart (H), or no RNA (N), and in the presence (+) or absence (−) of RT. RT-dependent products were only obtained from retina. (B) RNA used for cDNA synthesis was checked for integrity on a 1.5% agarose gel. 18S and 28S ribosomal RNA bands are clearly visible, indicating that both RNA samples were of good quality. Image published in: Moritz OL et al. (2002) Copyright © 2002. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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