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Fig. 4. A dominant negative Fzd7 induces cardia bifida phenotype. (A-Ai and F, Fi) Lateral and ventral views of wild type embryos at stage 29 showing normal tnnic (A, Ai) and nkx2-5 (F-Fi) expression in the heart. (B, Bi and G, Gi) Lateral and ventral views of embryos injected in the dorsal blastomeres at 4 cell stage with dominant negative fzd7 (fzd7 CRD). The cardia bifida phenotype is shown by tnnic (B, Bi) and nkx2-5 (G-Gi) expression. These embryos were fixed at the same stage as the control embryos in (A and F). (C) Graph showing fzd7 CRD cardia bifida phenotype percentages indicated by tnnic expression. (D, Di). Lateral and ventral views of embryos injected in the dorsal blastomeres (DB) at 4 cell stage with full length of fzd7 showing normal heart tube. Note that embryos in (D) and (G) are showing severe convergent extension defects but cardia bifida phenotype is only induced by fzd7 CRD. (E, Ei). Lateral and ventral views of injected embryo in the DB at 4 cell stage with fzd3 dominant negative form (fzd3 CRD) showing normal heart looping (at stage 38) indicating that fzd7 CRD cardia bifida phenotype is specific to Fzd7. Magnification 20x. (H and I). Lateral (H) and ventral (I) views of embryos injected in the DB at 4 cell stage with full length of fzd7 showing normal heart tube indicated by nkx2-5 expression. Image published in: Abu-Elmagd M et al. (2017) © 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue nkx2-5.L AR2, csx, Nkx-2.5, nkx-2.5, nkx2-5-a, nkx2-5-b, nkx2.5, tinman, XNkx-2.5, XNkx2-5 X. laevis Throughout NF stage 29 and 30 heart tnni3.L c-troponin, cardiac tropin T, cardiac troponin I, cTnI, ctnIc, cTNT, TIc, TnIc, tnnic, troponin-ic, XcTnI, XTnIc X. laevis Throughout NF stage 29 and 30 heart Image source: Published Larger Image Printer Friendly View

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