XB-IMG-159669
Xenbase Image ID: 159669
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Fig. 6. Functionality of froglet blastema epithelium for supporting limb development. (A) Schematic diagram of the experimental design. A developing limb bud was isolated from a st. 52 tadpole. Next, the epidermis was removed. The epidermis-stripped mesenchyme was grafted into a froglet blastema in the middle bud stage. To distinguish between host and graft cells, a transgenic Xenopus (H2B-GFP) was used as a graft. (B) Development of the grafted tissues in the regenerating froglet limb bud. The GFP positive graft was gradually patterned. (C) Phenotype of the grafted limb 1 month after surgery. (D) Skeletal pattern. (E) The section of the blastema containing the graft. The blastema was fixed and sectioned 5 days after the surgery. F and G are the higher magnifications of the adjacent section of the boxed region in E. (F) krt62.L expression was visualized by in situ hybridization. (G) The section shown in F was processed for immunofluorescence using anti-GFP antibody. GFP positive cells were derived from a graft. Scale bar in C and D = 1 mm. Scale bar in E = 500 µm. Scale bar in F= 100 µm. Image published in: Satoh A et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V.
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