Xenbase Image ID: 159689

Fig. S2. Folr1-MO or methotrexate does not induce apoptosis of neural plate cells during neurulation. (A) Two-cell-stage embryos were unilaterally injected with 10 pmol folate receptor 1 morpholino (Folr1-MO) and Alexa 594-conjugated dextran and grown until neural plate stage 17 (18.75 hpf). (B) Early neural plate stage embryos (stage 13, 14.75 hpf) were incubated in the absence (Control) or presence of 1-1.5 mM methotrexate (MTX) until closure of the neural tube in controls (stage 20, 21.75 hpf). (A,B) Embryos were then processed for TUNEL assay, immunostaining for β-tubulin and nuclear labeling with DAPI. Shown are representative transverse sections of neural tissue from aforementioned embryos. Positive control is a sample treated with DNase; negative control is a sample in which no labeling enzyme (Terminal Deoxynuclotidyl Transferase) was added. Scale bars: 20 μm. Image published in: Balashova OA et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue tubb.L beta5-tubulin, LOC108700239, tubb beta-5, tubb5, tubulin beta-1, XLOT X. laevis Throughout NF stage 17 neural plate tubb.L beta5-tubulin, LOC108700239, tubb beta-5, tubb5, tubulin beta-1, XLOT X. laevis Throughout NF stage 20 neural tube Image source: Published Larger Image Printer Friendly View

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