Xenbase Image ID: 159691

Fig. S4. Folr1 knockdown does not induce changes in actin dynamics or in overall apicobasal polarity in the folding neural plate. (A) Two-cell-stage embryos were unilaterally microinjected with Folr1-MO along with Alexa Fluor 594-dextran conjugate (red) and bilaterally injected with mCherry-UtropinCH (F-actin reporter, green). Superficial medial neural plate was time-lapse imaged from whole embryos at a rate of 1- 2 min−1. Fluorescence intensity profiles (arrows) across cell-cell borders were measured among wild-type (WT, 1) and Folr1-MO (2) containing cells during 15 min imaging. Scale bar, 10 μm. Intensity profiles were fitted with Gauss function using R software after background substraction. Bar graph shows mean±SEM peak width at 50% maximum intensity (left) and standard deviation (SD) of normalized maximum intensity during 15 min recording (right), n: 28 cell-cell borders per group, t-test. (B) Unilateral Folr1-MO-containing neural plate stage embryos were sectioned and processed for immunostaining with anti-ZO-1 (green). Scale bar, 20 μm. Image published in: Balashova OA et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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