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Figure 1. Effect of VPA on RD in transgenic X. laevis expressing P23H rod opsin. Top, Dot blot analysis of total rod opsin from whole eye extracts of P23H X. laevis (gray bars) and their WT siblings (black bars). VPA ameliorates RD significantly in a dose-dependent manner (ANOVA, p = 1.5 × 10−12). At 10 μm, the effect of VPA is equivalent to the effect of dark rearing. WT animals are unaffected by VPA (ANOVA, p = 0.363). p-values shown on chart are Dunnet's multiple comparisons. n = 10–17 animals per condition. Error bars indicate SEM. Bottom, Representative cryosections of contralateral eyes confirm that effects of VPA in dot blot assays are due to reduced RD in P23H-transgenic animals. OSs were stained with WGA (green) and Hoechst dye (blue). VPA treatment resulted in greater density of OSs in P23H X.laevis. WT animals were unaffected. Scale bar, 50 μm. Image published in: Vent-Schmidt RYJ et al. (2017) Copyright © 2017. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Experiment + Assay Source Phenotypes and Disease Xla.Tg(rho:hsa.rhosub_P23H) + NF48 (stained tissue microscopy) Fig.1.r1,c4 Anatomical Phenotype abnormal photoreceptor layer morphology abnormally decreased number of eye photoreceptor cell Disease retinitis pigmentosa Xla.Tg(rho:hsa.rhosub_P23H) + NF48 (Other Detection Assay) Fig1.graph Disease retinitis pigmentosa Expression Phenotype decreased amount rho.L expression in eye Larger Image Printer Friendly View

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