XB-IMG-159694
Xenbase Image ID: 159694
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Figure 2.
VPA ameliorates light-induced RD in a P23H rod opsin model. A, Dot blot analysis of P23H X. laevis and their WT siblings treated with 10 μm VPA in cyclic light and dark-reared conditions. In cyclic light, the effect of genotype and treatment were significant (two-way ANOVA, pg = 6 × 10−9, pt = 2 × 10−5) and VPA treatment significantly modified the effect of genotype (pi = 2 × 10−5). In the dark, the effect of genotype was significant (pg = 4 × 10−5), the effect of treatment was minimally significant (pt = 0.047), and treatment did not significantly modify the effect of genotype (p = 0.22). n = 5–13 animals per group. Error bars indicate SEM. B, Representative low-magnification confocal micrographs of cryosections from transgenic retinas expressing P23H rod opsin stained with WGA. Scale bar, 200 μm. C, Representative high-magnification confocal micrographs of transgenic retinas expressing P23H rod opsin stained with 2B2 anti-mammalian rhodopsin (green), WGA (red), and Hoechst dye (blue). Dark rearing promotes OS localization of P23H rhodopsin (arrowheads). VPA treatment does not alter P23H rhodopsin localization. ONL, Outer nuclear layer; INL, inner nuclear layer; IPL, inner plexiform layer. Scale bar, 50 μm. D, OS WGA and whole-retina 2B2 (P23H rhodopsin) signals were quantified in confocal microscopy images of dark reared transgenic animals. 2B2 signal was markedly reduced in both IS and OS of treated animals, whereas WGA signal was unchanged (two-way ANOVA pi = 3.5 × 10−9, t tests p = 7 × 10−5, p = 9 × 10−7, p = 0.004). Image published in: Vent-Schmidt RYJ et al. (2017) Copyright © 2017. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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