XB-IMG-159695
Xenbase Image ID: 159695
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Figure 3.
VPA exacerbates light-induced RD in a T17M rod opsin model. A, Dot blot analysis of VPA-treated T17M X. laevis and their WT siblings in cyclic light and dark-reared conditions. In cyclic light, the effects of genotype and treatment were significant (pg = 1.7 × 10−9, pt = 1.8 × 10−9) and VPA treatment modified the effect of genotype significantly (pi = 1.2 × 10−6). In the dark, there was no significant effect of genotype and no interaction between VPA and genotype (pg = 0.56, pt = 0.029, pi = 0.67). n = 6–16 animals per group. Error bars indicate SEM. B, Representative low-magnification confocal micrographs of cryosections from transgenic retinas expressing T17M rod opsin stained with WGA. Scale bar, 200 μm. C, Representative high-magnification confocal micrographs of transgenic retinas expressing P23H rod opsin stained with 2B2 anti-mammalian rhodopsin (green), WGA (red), and Hoechst dye (blue). Neither dark rearing nor VPA treatment altered the distribution of T17M rod opsin significantly. ONL, Outer nuclear layer; INL, inner nuclear layer; IPL, inner plexiform layer. Scale bar, 50 μm. D, Total rod opsin (B630N) and T17M rhodopsin (1D4) signals were quantified in dot blot analyses of dark-reared animals. Two-way ANOVA analysis showed no significant effects of treatment on antibody signals, indicating that VPA treatment did not alter T17M rhodopsin expression levels. n = 11–13 animals for each condition. Image published in: Vent-Schmidt RYJ et al. (2017) Copyright © 2017. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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