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Figure 7.
VPA treatment increases histone H3 acetylation in WT X. laevis eyes. A, Western blots of eye extracts probed with anti-acetyl H3, anti-H3, anti-acetyl tubulin, and anti-tubulin (top) were quantified (bottom) and showed an increase in relative levels of H3 acetylation on treatment with 10 μm VPA (p = 0.001, t test), but no significant change in relative levels of tubulin acetylation. n = 6–7 animals per condition. Error bars indicate SEM. B, Immunolabeling with anti-acetyl H3 (green) shows that the effect observed in A is due to increased anti-acetyl H3 labeling in all retinal layers, including photoreceptors. Blue indicates Hoechst 33342; red, WGA; ONL, outer nuclear layer; INL, inner nuclear layer; IPL, inner plexiform layer. Image published in: Vent-Schmidt RYJ et al. (2017) Copyright © 2017. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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