Xenbase Image ID: 159706

Fig. 5. β-catenin chromatin binding to Wnt-regulated genes. (A) β-catenin ChIP-seq of FG and HG explants identified 16,303 peaks within 20 kb (upstream or downstream) of 11,007 genes. The Venn diagram shows the overlap between 11,007 β-catenin-bound genes and 2991 Wnt-regulated genes (from Fig. 4B) grouped as FG enriched (n=524), HG enriched (n=304) or expressed at similar levels in the FG and HG (FG=HG; n=2163) based on RNA-seq data. BMP-activated genes are in red and BMP-repressed genes in blue. *P<0.05, hypergeometric test. (B) Overlap between 180 HG Wnt-activated genes (a), 208 FG Wnt-repressed genes (b), and genes with gain (left) or loss (right) of β-catenin ChIP enrichment upon BIO treatment. (C) Genome browser view of β-catenin peaks on HG-activated gene cdx2 and FG-repressed genes sfrp2 and nkx2-3. Red boxes indicate β-catenin significant peaks. (D,E) Average tag density of β-catenin (D) and p300 (E) peaks on HG Wnt-activated genes (a′) and two classes of FG Wnt-repressed genes (b′ and b′), comparing control with BIO treatment. *P<0.05, Wilcoxon test. (F) Motif enrichment analysis of β-catenin peaks. Image published in: Stevens ML et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page