XB-IMG-159707
Xenbase Image ID: 159707
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Fig. 6.
Smad1 and β-catenin converge on common CRMs. (A) Overlap of BMP-regulated/Smad1-bound genes with Wnt-regulated/β-catenin-bound genes. (B) Schematic of 33 genes with distinct β-catenin and Smad1 peaks and of 196 genes with overlapping β-catenin and Smad1 peaks. Right panel shows Smad1 and β-catenin read density in the corresponding peaks. WE, whole embryo. (C) Venn diagram showing the 196 genes with overlapping Smad1 and β-catenin peaks categorized based on activation (act) or repression (rep) by BMP and Wnt signaling. FG genes are in yellow and HG genes are in green. (D) Genome browser view of β-catenin and Smad1 peaks on hhex and cdx2 with illustration of CRMs tested in luciferase constructs. Red boxes indicate overlapping Smad1 and β-catenin peaks. (E) Motif enrichment analysis of gene sets based on BMP/Wnt regulation. (F) Luciferase assays of reporter constructs with CRMs depicted in D. FG cells were injected in the C1 blastomere and HG cells in the C4 blastomere of 32-cell stage embryos. Error bars represent s.d. of three biological replicates. *P<0.05, Student's t-test. Image published in: Stevens ML et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |