Xenbase Image ID: 159709

Fig. S1: Transcriptional program of FG and HG progenitors in vivo. (A) Scatter plot of log fold change (log2FC) in expression between HG versus FG samples and endo versus meso samples. HG-enriched transcripts (green), FG-enriched transcripts (orange) meso-enriched transcripts (red) and endo-enriched transcripts (yellow) based on log2 fold change (FC) ≤-1 or ≥1 and false discovery rate (FDR) ≤5%. (B) Xenopus orthologs of genes known to be involved in human and mouse GI development (manually curated list from the literature) are present in our FG-enriched and HGenriched gene lists. The heatmap shows that the Xenopus transcripts have restricted expression in manner predicted from the mouse and human literature, illustrating high conservation across species. (C) GO term enrichment analysis of 172 FG-endo, 294 FG-meso, 518 HG-endo and 202 HG-meso genes from Fig. 1C. (D) BMP and Wnt pathway components that are expressed in any sample (FG-endo, FGmeso, HG-endo or HG-meso) above one transcripts per-million reads (TPM >1; lower than this is considered not expressed). The heatmap shows that BMP pathway genes are expressed in both the FG and HG, whereas Wnt ligands are generally restricted to the HG and Wnt-antagonists enriched in the FG. (E) In situ hybridization of mid-sagittal section stage NF20 (hhex and ventx2.1) or NF35 embryos (nr1h5, nkx2-1, nkx2-5, sox2 and darmin) in DMH1 or Tg(hsp70:dkk1) embryos; anterior left and dorsal up. Image published in: Stevens ML et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue hhex.L hex, tHex, XHex X. laevis Throughout NF stage 20 foregut mesoderm endoderm ventx2.L ventx2.1, ventx2.1-a, ventx2.1-b, vox, vox1, Xbr-1a, xvent-2, xvent-2b, Xvr-11b X. laevis Throughout NF stage 20 hindgut mesoderm endoderm circumblastoporal collar nr1h5.L for1 X. laevis Throughout NF stage 35 and 36 foregut endoderm liver nkx2-1.L nkx2.1, titf1, ttf1, Xnkx-2.1, xnkx2-1 X. laevis Throughout NF stage 35 and 36 endoderm lung bud forebrain hindbrain nkx2-5.L AR2, csx, Nkx-2.5, nkx-2.5, nkx2-5-a, nkx2-5-b, nkx2.5, tinman, XNkx-2.5, XNkx2-5 X. laevis Throughout NF stage 35 and 36 foregut heart pharyngeal region sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 35 and 36 foregut spinal cord head brain pharyngeal region darmin.L cyl18, endocut, glutamate carboxypeptidase-like protein 1 X. laevis Throughout NF stage 35 and 36 hindgut endoderm midgut Image source: Published Larger Image Printer Friendly View

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