Xenbase Image ID: 159711

Fig. S3: Smad1 ChIP-seq of embryos stage NF20. (A) Genomic distribution of Smad1 ChIP-seq peaks in stage NF20 embryos categorized as upstream (- 20kb), downstream (+20kb), intragenic and promoter (-1kb to +1kb) regions. (B) Smad1 ChIP-PCR of known BMP-target genes showing reduced Smad1-binding to CRMs of msx1, id3 and ventx2.1 promoters in DMH1 treated embryos compare to DMSO controls. (C) Peak overlap between Smad1 and p300 ChIP-seq of stage NF20 whole embryos. (D) Venn showing the overlap between Smad1-bound genes, p300-bound genes and genes expressed in NF20 embryo at levels higher than 5 transcripts per million (TPM>5) based on RNA-seq. (E) Motif enrichment analysis of Smad1 ChIP-seq peaks associated with activated (act) or repressed (rep) genes. Image published in: Stevens ML et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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