XB-IMG-159712
Xenbase Image ID: 159712
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Fig. S4: RNA-seq of control, Tg(hsp70:dkk1) and BIO treated embryos.
(A) Venn diagram illustrates transcripts with expression increased (é) or decreased (ê) upon
Tg(hsp70:dkk1) or BIO treatment in FG or HG tissues. Wnt-activated genes were log2FC ≤-1 upon heatshock
or log2FC ≥1 upon BIO treatment FDR ≤5% (n=959). Wnt-repressed genes were log2FC ≥1 upon heat-shock or log2FC ≤-1 upon BIO treatment FDR ≤5% (n=2032). Forty-one transcripts had ambiguous
regulation with evidence of being both Wnt-activated and Wnt-repressed, and were excluded from further
analysis. Overall we categorized a total of 2991 (959+2032) Wnt-regulated genes in the FG and HG
tissue. (B) GO term enrichment analysis of Wnt-activated and Wnt–repressed genes. (C-D) Scatter plot
showing log2FC in expression between FG non-heatshock and FG Tg(hsp70:dkk1) (C) and HG DMSO
and HG BIO (D) explants. Transcripts are colored based on the normal control expression; HG-enriched
in green, FG-enriched in orange, expressed similarly FG and HG (FG=HG) in grey and normally not
expressed in FG or HG in black. Image published in: Stevens ML et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |