XB-IMG-159717
Xenbase Image ID: 159717
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Fig. 2. Transgenic embryos and larvae carrying the
DEVD reporter show dynamic spatio-temporal GFP
expression patterns during their development.
(A) Dorsal and (B) anterior view of a stage 18–19
X. tropicalis embryo at neural fold stage. GFP expression
emerges in defined stripes on each side of the dorsal
midline, which are most intense in the future brain region
as well as in the anterior patches corresponding to the eye
vesicle. (C) Similar patterns are visible at stage 24 of a
X. tropicalis tailbud embryo. (D–J) A dynamic and
diversified GFP expression signal is observed in reporter
embryos as development proceeds. Particularly high
expression levels were observed in the developing neural
tissues. (F) Stage 46 X. laevis tadpoles show GFP
positivity in kidney cells (closer view in H), the brain, nasal
pits (closer view in G) and muscle fibers (I). (J) The lens
was still GFP positive. (K–L) Around metamorphosis
(X. laevis, stages 60–64), GFP is expressed in the growth
zones of the long bones. (M) The teeth and some cranial
structures, including the skull and the jawbone, were also
GFP positive. b, brain; bo, bone; e, eye; ev, eye vesicle;
he, heart; k, kidney cells; mf, muscle fibers; np, nasal pits;
nt, neural tube; sc, spinal cord; t, teeth. Image published in: Tran HT et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, The Company of Biologists Ltd. Larger Image Printer Friendly View |