XB-IMG-159730
Xenbase Image ID: 159730
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Fig. 1. Intracellular Ca2+ is required for
Xenopus NTC. (A) Dorsal views of embryos at
stage 16 treated with DMSO, 25 μM 2APB and
200 μM nifedipine. Dashed lines indicate the
outlines of the NP. (B) The mean width of the
NP in embryos, as measured by visualizing the
expression of the pan-neural marker gene
Sox2. Error bars depict s.e.m. The number of
embryos examined is shown on each bar.
**P<0.01 and ***P<0.001 compared with the
DMSO-treated control; two-sided Welch’s
t-test. (C) Transverse sections of stage-16
embryos stained with phalloidin (top) and
outlines of neural tissues and cells (bottom).
Scale bars: 100 μm. (D) Apical width of stage-
16 embryos. Black line indicates the median
value. **P<0.01 and ***P<0.001, two-sided
Mann–Whitney U-test; n=54 cells, nine
embryos (DMSO); 97 cells, 12 embryos
(2APB); 88 cells, 12 embryos (nifedipine).
(E) In situ hybridization analysis of inhibitor-treated
embryos. Dorsal views showing the
expression of Sox2, a pan-neural marker (top),
N-tubulin (tubb2b), which marks differentiated
neurons (middle), and Epidermal keratin (Epi.
keratin), an epidermal marker (bottom). Anterior
is to the top. The expression patterns were
similar in inhibitor-treated embryos and DMSOtreated
controls, but the expression domains
were wider in the inhibitor-treated embryos
because of delayed NTC. Image published in: Suzuki M et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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