XB-IMG-159731
Xenbase Image ID: 159731
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Fig. 2. Live-imaging analyses of intracellular Ca2+ in
the neural plate. (A) A Ca2+ transient at the single-cell
level, visualized with R-GECO1.0 (gray), in a dorsal
view of the presumptive anterior spinal cord region;
anterior is to the top. R-GECO1.0 was introduced on the
left side of the embryo. The midline is indicated by a
dotted line. A time course over a 40 s period of four
sample cells (which are indicated in the figure) is shown
in the right panels. Scale bar: 100 μm. (B) Time-lapse
imaging data of a multicellular, wave-like Ca2+ transient
in the embryo shown in A. (C) Fluorescence intensities
of cytoplasmic R-GECO1.0 over time; the shaded area
indicates the s.d. In multicellular Ca2+ transients (red),
cells adjacent to the cell that was initially activated were
measured (n=6 cells). (D) The radius and velocity of
wave propagations in multicellular Ca2+ transients. The
red line represents the relationship between the two
data sets, calculated using the least-squares method of
linear regression (n=21 cells, five embryos). Image published in: Suzuki M et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |