XB-IMG-159733
Xenbase Image ID: 159733
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Fig. 4. Extracellular ATP in Ca2+ fluctuations and NTC. (A) Mean number of
Ca2+ transients in embryos expressing an E-NTPDase1 mutant (δACR; red) or
E-NTPDase1 (NTPDase1; green), shown as smoothed curves obtained by
averaging the originally counted values at a given time point with those of
neighboring time points (25 time points total). Time interval of observations is
40 s. Shaded area indicates the s.d. (B) Number of single-cell Ca2+ transients
in the early and late phases of NTC. *P<0.05, two-sided Mann–Whitney U-test.
(C) Number of multicellular Ca2+ transients in the early and late phases of NTC.
*P<0.05, two-sided Mann–Whitney U-test. (D) The mean cross-correlation
between the closing speed and the total R-GECO1.0 value in embryos
expressing a mutant E-NTPDase1 (δACR; red) or E-NTPDase1 (NTPDase1;
green). The shaded area indicates s.d. n=3 (δACR), 5 (NTPDase1) embryos.
Results in A-D are for the same data set. (E) Images showing the yellow-tocyan
(yellow/cyan; Y/C) fluorescence ratio value of AT3.10RK-GPI (top,
control), AT3.10-GPI (second to bottom) on the surface of NP cells, by FRET
analysis (left). The mean ratio was measured from the masked region
generated by membrane-targeted mRFP (right). Scale bar: 30 μm. (F) The
mean yellow-to-cyan fluorescence ratio value of AT3.10-GPI on cell surfaces in
the NP by FRET analysis. The number of embryos examined is indicated in
each bar. Error bars show s.e.m. **P<0.01 and ***P<0.001, two-sidedWelch’s
t-test. n.s., not significant. In B and C, the box and whiskers of these plots
indicate maximum, third quarter, median, first quarter and minimum values of
each groups, respectively, from upper side to the bottom of the graphs. Image published in: Suzuki M et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |