Xenbase Image ID: 159738

Figure S2. Method of the live-imaging analyses of neural tube closure. (A) Schematic of Xenopus embryo microinjection and confocal experiments. GECO RNAs were injected into the presumptive neural region in embryos at the four-cell stage. The embryos were then cultured to the appropriate developmental stage, after which the vitelline membranes were removed. The embryos were mounted in a hand-made agarose gel chamber on a glass-base dish filled with 0.3x MMR, and were observed by an inverted microscope equipped with a spinning-disk confocal unit and an EM-CCD camera. (B) Workflow for the quantitative imaging analyses of Ca2+ dynamics in the neural plate (see Materials and Methods for the detail.) Image published in: Suzuki M et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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