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Figure S3. Supplementary profiles of Ca2+ fluctuation. (A) The number of Ca2+ transients occurring during NTC in the individual embryo shown in Fig. 3. Plots are shown as smoothed curves of the original counts by averaging values at a given time point with neighboring time points (50 time points total). (B) The mean yellow/cyan fluorescence ratio of the FRET-based Ca2+ indicators YC-Nano50, YC-Nano30, and YC-Nano15 in the neural plate in embryos treated with calcium-channel inhibitors. Error bars depict s.d. The number of embryos examined is shown on each bar. Statistical significances were assessed by two-sided Student’s t test. (C) The number of Ca2+ transients during NTC in the embryo shown in Fig. 4. The plot is shown as smoothed curves obtained by averaging the originally counted numbers at a given time point with those of neighboring time points (25 time points total).

Image published in: Suzuki M et al. (2017)

Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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