Xenbase Image ID: 159767

Fig. 2. Knockdown ofaqp3bresults in defects during gastrulation.Xenopus embryos were pre-injected at the 1-cell stage with lacZ RNA (injection control) (A-H) or aqp3bMM RNA, which is not recognized by either aqp3b MO (I, J). At the 4-cell stage, the embryos were additionally injected into one dorsal blastomere with control MO1 (A, E), aqp3b MO1 (C, G, I), control MO2 (B, F), or aqp3b MO2 (D, H, J) together with the injection tracer rhodamine-dextran. (Each embryo set is paired with the corresponding fluorescent images.) Blastopore closure proceeded normally in control MO-injected embryos (A, B, E, F). aqp3b MOs did not have gross effects on early blastopore closure in stage 10.5 embryos (C, D), but clear defects developed by stage 12 (G, H), which were rescued by aqp3bMM RNA (I, J). (K) Percentage of embryos showing gastrulation defects at stage 12 (injection as above with aqp3b MOs, control MOs, or rescue of aqp3b MO defects with aqp3bMM RNA). Error bars represent standard error of the mean. Seven independent clutches were scored with a total number of embryos as indicated. (L) In four independent experiments, 40–90 embryos were scored per clutch for the types of phenotypic defects resulting from aqp3b MO1 injection and rescue, compared to control MO1 injections. Image published in: Forecki J et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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