Xenbase Image ID: 159768

Fig. 3. Impairedaqp3bexpression results in abnormal tissue architecture.aqp3b MO1-injected embryos (stage 10.5) were analyzed by confocal microscopy to visualize phalloidin (green) and co-injected rhodamine-dextran tracer (red). Embryos that received control MO1 in their dorsal marginal zone (A, B) showed a highly organized boundary between ectoderm (e) and mesendoderm (me), which was revealed by actin cytoskeletal staining (A). In contrast, aqp3b MO1-injected embryos (C, D) displayed irregularities in this boundary and jagged protrusions were found along the ectoderm layer (arrows, C). In higher magnification images, control MO1-injected embryos (E, F) showed highly organized tissue architecture and regularly shaped cells, while in aqp3b MO1-injected embryos (G, H), the ectoderm/mesendoderm boundary was irregular and cells seemed less tightly packed (arrows). dlb, dorsal lip of the blastopore; e, ectoderm; me, mesendoderm. Scale bars: 50um. Image published in: Forecki J et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue actb.L beta actin, beta-actin, g-actin X. laevis Throughout NF stage 10.5 upper blastopore lip dorsal marginal zone endomesoderm ectoderm Image source: Published Larger Image Printer Friendly View

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