XB-IMG-159773
Xenbase Image ID: 159773
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Fig. 8.
Convergence but not radial intercalation movements were affected in embryos that receivedaqp3bMO. Embryos co-injected with both aqp3b MOs (B, D) showed equal thinning of the blastocoel roof as embryos that received both control MOs (A, C); stage 9 (A, B), stage 11 (C, D). Similarly, stage 11–11.25 embryos injected with control MOs (E, F) or with aqp3b MOs (G, H) showed equally thinned DMZs (arrowheads), indicating normal radial intercalation. Embryos were stained with antibodies against β-catenin (red) to outline cells (E-H) and with anti-EGFP antibodies (green) to indicate areas that received the aqp3b MOs (A-H). In situ hybridization with chordin probe of mid-gastrula embryos (stage 11; I-L) and after completion of gastrulation (stage 13; M-P) demonstrated that uninjected (I, M) and control MOs-injected (J, N) embryos showed normal convergent extension movements. Wider chordin expression patterns in aqp3b MOs-injected embryos (K, L, O, P) were consistent with defects in convergence movements. The defects ranged from mild (O) to strong (P), which is quantified in (Q). Four independent batches were scored with a total number of embryos as indicated. Tailbud embryos (R-T) showed shortened body axes when injected with aqp3b MOs (T). Arrows indicate the injected sides of the embryos. The embryo in (L) contained remnants of wild type pigment. Thus, brackets indicate the extent of chordin expression. Scale bars: 25 um (A-D); 100 um (E-H). Image published in: Forecki J et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, Elsevier B. V.
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