Xenbase Image ID: 1682

Figure 6. Effects of FoxN3 depletion on neural crest formation. Embryos were unilaterally injected with 13 ng FoxN3-MO and 500 pg GFP RNA. After sorting for right/left injections, embryos were submitted at different developmental stages to in situ whole mount hybridization. The asterisks indicate the side of injection. A: FoxD3, stage 23. B:AP2 alpha , stage 23. C:snail, stage 23. D:AP2 alpha , stage 29. E:snail, stage 29. No difference in the expression domains between injected and uninjected sides can be observed. F,G: Whole mount in situ hybridization of unilaterally injected embryos stained for HoxA2 (F) and Sox3 (G). Embryos were injected at their right sides. H-K: Whole mount in situ hybridization of bilaterally FoxN3-MO or Co-MO-injected embryos (13 ng pro blastomere). H:Xbap, Co-MO-injected, stage 38. I:Xbap, FoxN3-MO injected, stage 38. Xbap expression is visible close to the cement gland in the controls (see arrow). The FoxN3-MO-injected embryos show reduced expression of Xbap in the prospective jaw (see arrow). The ventral part of this expression domain is completely absent. J:Xbap, Co-MO-injected, stage 43, ventral view. K:Xbap, FoxN3-MO-injected, stage 43, ventral view. The Xbap expression in the controls is restricted at stage 43 within the head to Meckel's cartilage. FoxN3-MO-injected embryos show severe reduction of staining (see arrows). Image published in: Schuff M et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue snai1.S LOC108702592, sna, snail, snail1, xSna, Xsnail X. laevis Throughout NF stage 23 neural crest neural plate tfap2a.L ap2, AP2a, AP2alpha, tfap2a-a, tfap2a-b, tfap2alpha, XAP-2, xap2 X. laevis Throughout NF stage 23 periocular region cranial neural crest sox3.S Sry, Xlsox3, xSox3, Xtsox3 X. laevis Sometime during NF stage 28 to NF stage 32 otic vesicle olfactory placode lateral line placode lens brain mandibular crest hyoid crest anterior branchial crest posterior branchial crest intermediate mesoderm cranial placode hoxa2.L hox1k, hoxa2-a, hoxa2-b, hoxa2b, X-Hoxa2 X. laevis Sometime during NF stage 28 to NF stage 32 hindbrain mandibular crest hyoid crest posterior branchial crest anterior branchial crest nkx3-2.S bagpipe homeobox homolog 1, bapx1, nkx3.2, Xbap X. laevis Sometime during NF stage 37 and 38 to NF stage 43 pharyngeal arch foregut lateral plate mesoderm cartilage tissue Meckel's cartilage optic vesicle foxd3.L fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 X. laevis Throughout NF stage 23 cranial neural crest neural crest eye primordium snai1.S LOC108702592, sna, snail, snail1, xSna, Xsnail X. laevis Throughout NF stage 23 cranial neural crest neural plate border eye primordium tfap2a.L ap2, AP2a, AP2alpha, tfap2a-a, tfap2a-b, tfap2alpha, XAP-2, xap2 X. laevis Throughout NF stage 29 and 30 cranial neural crest periocular region head mesenchyme snai1.S LOC108702592, sna, snail, snail1, xSna, Xsnail X. laevis Throughout NF stage 29 and 30 eye primordium brain cranial neural crest Image source: Published Larger Image Printer Friendly View

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