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Fig. 4. Expression of known zygotic endoderm regulators in Nodal-, Mixer- and Sox17- embryos. The expression of the known endoderm regulators Xnr1, Xnr2, Xnr4, Mix1, Mix2, Mixer, Bix1, Bix2, Bix3, Bix4, Gata4, Gata5, Gata6, Sox17α, Sox17β, Edd and Hnf1β from (A) the microarray and (B) real-time RT-PCR validation was determined from Nodal- (Cerberus-S injected 1 ng/embryo), Mixer- (Mixer morpholino injected 40 ng/embryo) and Sox17- (Sox17α1+α2+β morpholino injected 20 ng each/embryo) embryos at stage 11. Both the array data and RT-PCR is plotted on a log scale. Changes in expression less than 1.4-fold up or down from the control are within the horizontal yellow bar. From these and the validation of more than 40 other genes changes in 1.4-fold were considered robust and were validated in over 75% of the cases. Image published in: Sinner D et al. (2006) Copyright © 2006. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Experiment + Assay Source Phenotypes and Disease Xla Wt + cer1del + NF11 (microarray) fig.4.a Expression Phenotype decreased amount expression in embryo decreased amount a2m.L expression in embryo decreased amount bix1.1.L expression in embryo decreased amount bix1.2.L expression in embryo decreased amount bix1.3.L expression in embryo decreased amount gata4.L expression in embryo decreased amount gata5.L expression in embryo decreased amount gata6.L expression in embryo decreased amount hnf1b.L expression in embryo decreased amount mix1.L expression in embryo decreased amount mixer.L expression in embryo decreased amount nodal1.L expression in embryo decreased amount nodal2.L expression in embryo decreased amount sox17a.L expression in embryo decreased amount sox17b.2.L expression in embryo Xla Wt + mixer MO + NF11 (microarray) fig.4.a Expression Phenotype increased amount expression in embryo decreased amount a2m.L expression in embryo increased amount bix1.1.L expression in embryo increased amount bix1.2.L expression in embryo increased amount bix1.3.L expression in embryo decreased amount gata4.L expression in embryo decreased amount gata5.L expression in embryo decreased amount gata6.L expression in embryo decreased amount hnf1b.L expression in embryo increased amount mix1.L expression in embryo increased amount mixer.L expression in embryo increased amount nodal.L expression in embryo increased amount nodal1.L expression in embryo increased amount nodal2.L expression in embryo Xla Wt + sox17a MO + sox17b.1 MO + NF11 (microarray) fig.4.a Expression Phenotype decreased amount a2m.L expression in embryo decreased amount bix1.1.L expression in embryo decreased amount bix1.2.L expression in embryo decreased amount bix1.3.L expression in embryo decreased amount gata4.L expression in embryo decreased amount hnf1b.L expression in embryo decreased amount mix1.L expression in embryo decreased amount nodal1.L expression in embryo increased amount sox17a.L expression in embryo increased amount sox17b.2.L expression in embryo Xla Wt + cer1del + NF11 (RT-PCR) fig.4.b Expression Phenotype decreased amount expression in embryo decreased amount a2m.L expression in embryo decreased amount bix1.1.L expression in embryo decreased amount bix1.2.L expression in embryo decreased amount bix1.3.L expression in embryo decreased amount gata4.L expression in embryo decreased amount gata5.L expression in embryo decreased amount gata6.L expression in embryo decreased amount hnf1b.L expression in embryo decreased amount mix1.L expression in embryo decreased amount mixer.L expression in embryo decreased amount nodal.L expression in embryo decreased amount nodal1.L expression in embryo decreased amount nodal2.L expression in embryo decreased amount sox17a.L expression in embryo decreased amount sox17b.2.L expression in embryo Xla Wt + mixer MO + NF11 (RT-PCR) fig.4.b Expression Phenotype decreased amount a2m.L expression in embryo increased amount bix1.1.L expression in embryo increased amount bix1.2.L expression in embryo increased amount bix1.3.L expression in embryo decreased amount gata4.L expression in embryo decreased amount gata5.L expression in embryo decreased amount hnf1b.L expression in embryo increased amount mix1.L expression in embryo increased amount nodal1.L expression in embryo increased amount nodal2.L expression in embryo Xla Wt + sox17a MO + sox17b.1 MO + NF11 (RT-PCR) fig.4.b Expression Phenotype decreased amount a2m.L expression in embryo decreased amount bix1.1.L expression in embryo decreased amount bix1.2.L expression in embryo decreased amount bix1.3.L expression in embryo decreased amount gata4.L expression in embryo decreased amount gata5.L expression in embryo decreased amount hnf1b.L expression in embryo decreased amount mix1.L expression in embryo decreased amount nodal1.L expression in embryo Larger Image Printer Friendly View

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