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Figure 3. Effect of oocytes development on the organization of the nuclear pore complexes. (A–C) 2D histogram of the probability P(d,α) to observe a NPC on a given envelope with two neighbors at a distance d and forming an angle α respectively for oocytes at stage II, IV and VI. For stage VI oocytes the most probable coordinates are (135 ± 5 nm, 90 ± 6°). The frequency event is normalized by the maximum for each histogram. (D–F) First neighbor angle distribution evolution for respectively stage II, stage IV and stage VI oocytes. The red dashed line at 60° corresponds to the minimal angle possible between 3 NPCs. (G) Experimental most probable angle α* between three nuclear pore complexes as a function of the distance d between the central pore and its two neighbors (dashed line) and theoretical angle α between three nuclear pore complexes as a function of the distance d between the central pore and its two neighbors in the packing model (solid line). In this model all the nuclear pore complexes are in contact with their first neighbors but without any large-scale order. Image published in: Sellés J et al. (2017) © The Author(s) 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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