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Figure 6. Consensus phosphorylation sites. GproX was used for unsupervised and unstandardized clustering of all identified phosphopeptide sequences. Upper and lower threshold log2 values of 0.26 and −0.32 were used, corresponding to ratios of 1.2 and 0.8, respectively. This generated 22 unique clusters according to changes in phosphorylation as a function of developmental time. Expression profile and corresponding consensus sequences generated using WebLogo for (A) cluster 20 and (B) cluster 21. Image published in: Peuchen EH et al. (2017) © The Author(s) 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |