Xenbase Image ID: 168549

Figure 1. Depletion of XTRPM6 caused gastrulation defects during Xenopus embryogenesis. (a) Temporal expression pattern of XTRPM6. The expression level of XTRPM6 RNA was assessed by RT-PCR analysis. ODC was used as an internal control. RT(-) is without reverse transcriptase as a negative control. Full gel images shown in Supplementary Figure 5. (b) Spatial expression pattern of XTRPM6. Whole-mount in situ hybridization was performed using an XTRPM6 anti-sense RNA probe. No signal was detected using an XTRPM6 sense RNA probe. Arrowheads for stage 17 (dorsal) indicate lateral staining; the dotted line indicates where the transverse section was done. Arrowhead for stage 17 (anterior) indicates strong anterior staining overlapping with the cement gland and the pre-placodal region. Arrowheads for stage 23 and stage 28 indicate XTRPM6 expression in primary heart field and pronephric duct, respectively. St: stage. (c) TRPM6 is required for gastrulation. A control MO or XTRPM6 MO was injected into the two dorsal or ventral blastomeres at the 4-cell stage, and the phenotype was observed at tadpole stages. Dorsal injection of XTRPM6 MO or XTRPM7 MO caused gastrulation defects. Ventral injection of XTRPM6 MO but not XTRPM7 MO caused a shortened and curved axis. For the rescue experiments, 40 ng of the XTRPM6 MO was co-injected with the specified mRNAs. (d) Quantification of phenotypes scored at stage 28–30. The collective total number of injected embryos from all experiments is indicated above each bar. Image published in: Komiya Y et al. (2017) © The Author(s) 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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