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Figure 4. XTRPM6 but not XTRPM7 is required for radial intercalation. (a) Control and XTRPM6 MOs were injected into the animal pole at 2-cell stage embryo. LacZ RNA was co-injected with MO as a tracer. At late gastrula stage (stage 12-13) the process of blastopore closure was observed (upper panels). Embryos were stained for β-galactosidase activity using Red-gal as a substrate (lower panels) to visualize the distribution of LacZ-positive cells. No defect in blastopore closure was observed in XTRPM6 MO-injected embryos. (b) Control and XTRPM6 MOs were laterally injected into two ventral blastomeres with LacZ RNA at 4-cell stage embryo. Embryos were stained for β-galactosidase activity at stage 14–15. XTRPM6 MO injected embryos had very little staining on the embryo surface compared to control MO-injected embryos. (c) and (d) Red-gal stained embryos injected with the XTRPM6 MO or control MO were partially dissected. For control MO-dissected embryos, strong staining was observed in the outer epithelial ectoderm layer of the embryo (indicated with brackets). In contrast, strong staining was not readily observed at the outer epithelial ectoderm layer (indicated with brackets) for TRPM6 MO-dissected embryos; instead strong staining was observed in deeper layers of the embryo and on the archenteron roof. (e) Quantification of surface staining from (b). The collective total number of injected embryos from all experiments is shown above each bar. Error bars indicate standard error. **P < 0.01. Image published in: Komiya Y et al. (2017) © The Author(s) 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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