Xenbase Image ID: 1694

Fig. 3. Tsg and Bmp7 cooperate in ventral development in Xenopus embryos. (A,F,K,P) Control and morpholino-injected embryos at stage 12 hybridized with sizzled probe. Expression of sizzled is reduced in Bmp7-MO- or Tsg-MO-injected embryos and almost completely lost in the Bmp7-MO/Tsg-MO-injected embryos. (B,G,L,Q) Expression of Otx2 in injected embryos at stage 13; there is an increase in Bmp7-MO/Tsg-MO-injected embryos. (C,H,M,R) Embryos at stage 18 hybridized with Otx2 and Myod1 probes. The expansion of Otx2 regulates and is no longer seen at this stage. (D,E,I,J,N,O,S,T) Phenotypes of morpholino-injected embryos at (D,I,N,S) stage 39 (2 day) and (E,J,O,T) stage 42 (3 day). Bmp7-MO-injected embryos have a reduced ventral fin (arrowhead); Tsg-MO-injected embryos have a bent tail and reduction of ventral fin. Co-injection of Bmp7-MO and Tsg-MO synergize, causing a severe loss of ventral fin (arrow in S) and at later stages truncation of the entire tail region (T). Image published in: Zakin L et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue szl frzb3, secreted frizzled, sizzled, xszl Xenopus Throughout NF stage 12 ventral ventral marginal zone otx2 otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 Xenopus Sometime during NF stage 12 to NF stage 18 anterior anterior neural fold eye primordium myod1 mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD Xenopus Throughout NF stage 18 mesoderm presomitic mesoderm Image source: Published Larger Image Printer Friendly View

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