Xenbase Image ID: 169541

Figure 4. Pk3 depletion prevents γ-tubulin and Nedd1 recruitment to the basal body.Embryos were injected with 0.2 ng of RNA encoding GFP (A–D) or Histone-GFP (E,F) as a lineage tracer and CoMO (A,C,E) or Pk3MO1 (B,D,F) (15 ng each). GRP explants from stage 17 embryos were double stained for GFP and γ-tubulin (A,B), Nedd1 (C,D) or Centrin1 (E,F). En face staining is shown, anterior is at the top. Representative images from three to five independent experiments are shown, with 6–10 explants examined per group. The effects of Pk3MO1 on γ-tubulin and Nedd1 are detectable in the majority of cells depleted of Pk3 (n > 100). Image published in: Chu CW et al. (2016) Copyright © 2016, Macmillan Publishers Limited. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Experiment + Assay Source Phenotypes and Disease Xla Wt + prickle3 MO + NF17 (immunohistochemistry) fig.4.b,b^1 Anatomical Phenotype abnormal ciliary basal body organization Expression Phenotype decreased amount tubg1.L expression in basal body Xla Wt + prickle3 MO + NF17 (immunohistochemistry) fig.4.d.d^1 Expression Phenotype decreased amount nedd1.L expression in basal body Larger Image Printer Friendly View

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