XB-IMG-169571
Xenbase Image ID: 169571
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Figure 2. Chd7 is required for proper CNS myelination(a) Diagram depicting Cre mediated excision of the floxed Chd7 exon 2.(b) Immunolabeling of Chd7 and Olig2 in the corpus callosum of P14 brain from control and Chd7cKO mice. Scale bar, 40 μm.(c) mRNA expression of Mbp in the spinal cord of control and Chd7cKO mice at P0, P7 and P14. Scale bars, 100 μm. Arrows point to the spinal white matter region.(d) mRNA expression of Plp in P7 and P14 brain sections from control and Chd7cKO mice. Scale bar, 100 μm. Arrows point to the white matter.(e) Immunostaining for CC1 and Olig2 in the corpus callosum from control and Chd7cKO mice at P7. Scale bar, 100 μm.(f) Quantification of CC1+ OLs as a percentage of total Olig2+ cells in the corpus callosum of Control and Chd7cKO at indicated ages (The data are presented as mean ± s.e.m. For P9, n = 3 controls and 2 mutants, * p = 0.026, t = 4.12; for P15, n = 4 controls and 4 mutants, ** p = 0.009, t = 3.79; n, numbers of animals; Two-tailed unpaired Student’s t test).(g) Immunolabeling of MBP on coronal brain sections from control and Chd7cKO mice at P9. Nuclei are counterstained with 4,6-diamidino-2-phenylindole (DAPI; blue). Scale bar, 1 mm.(h) Quantification of total cerebral white matter volume (MBP+) of control and Chd7cKO mice at P9 and P15 (The data are presented as mean ± s.e.m. For P9, n = 3 controls and 3 mutants, ** p = 0.002, t = 7.22; for P15, n = 4 controls and 4 mutants, ** p = 0.009, t = 3.74; n, numbers of animals; Two-tailed unpaired Student’s t test).(i,j) Electron micrographs of transverse optic nerve sections from P14 (i) and P28 (j) control and Chd7cKO mice. Scale bars, 4 μm.(k) Quantification of myelinated axons as a percentage of total axons (The data are presented as mean ± s.e.m. For P14, n = 3 controls and 3 mutants, ** p = 0.0017, t = 10.73; for P28, n = 4 controls and 4 mutants, * p = 0.0217, t = 3.656; n, numbers of animals; Two-tailed unpaired Student’s t test).(l) Quantification of myelin sheath thickness (g-ratio) in control and Chd7cKO optic nerves (p < 0.0001; for control, g-ratio = 0.7227 ± 0.007414, n = 196 axons from 3 control optic nerves; for Chd7cKO, g-ratio = 0.8325 ± 0.003235, n = 202 axons from 3 Chd7cKO optic nerves; Two-tailed unpaired Student’s t test). Image published in: He D et al. (2016) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View |