Xenbase Image ID: 169573

Figure 4. Chd7 is required for timely CNS remyelination(a,b) Immunostaining showing Chd7 and MBP at Dpl 7 and 14 in spinal cord LPC lesions from 8-week old mice. Scale bar, 50 μm.(c) Immunostaining showing Chd7, CC1, and Sox10 at Dpl 14 in spinal cord LPC lesions. Scale bar, 50 μm.(d) In situ hybridization for Mbp and Plp and immunolabeling for PDGFRα and Olig2 in spinal LPC lesions of control and Chd7cKO mutants at Dpl 7 and 14. Scale bars, 100 μm.(e,f) Quantification of Plp1+ OLs and PDGFRα+ OPCs in LPC lesion sites at Dpl 7 and 14 (The data are presented as mean ± s.e.m. For Plp1 at Dpl 7, n = 4 controls and 4 mutant animals, * p = 0.022, t = 3.079; for Plp1 at Dpl 14, n = 4 controls and 4 mutant animals, ** p = 0.0098, t = 3.726; for PDGFRα+ OPCs at Dpl 7, n = 4 controls and 3 mutant animals, p = 0.848, t = 0.202; for PDGFRα+ OPCs at Dpl 14, n = 3 controls and 4 mutant animals, p = 0.299, t = 1.158; Two-tailed unpaired Student’s t test).(g) Immunostaining for CC1 in LPC lesions from control and Chd7cKO spinal cords at Dpl 14. Scale bar, 100 μm.(h) Quantification of CC1+ OLs in LPC lesion sites at Dpl 14 (n = 4 controls and 4 mutant animals, ** p = 0.0015, t = 5.523; whiskers show minimum and maximum, box limits are first and third quartile and center lines are median; Two-tailed unpaired Student’s t test)(i) EM images of LPC lesions from control and Chd7cKO spinal cords at Dpl 14. Scale bar, 2 μm.(j) The percentage of remyelinated axons in LPC-induced lesions of control and Chd7cKO spinal cords at Dpl 14 (n = 4 controls and 4 mutant animals, * p = 0.0188, t = 3.191; whiskers show minimum and maximum, box limits are first and third quartile and center lines are median; Two-tailed unpaired Student’s t test).(k) The myelin g-ratio in LPC-induced lesions of control and Chd7cKO mutants at Dpl 14 (for control, g-ratio = 0.8943 ± 0.005128, n = 128 remyelinated axons from 4 control mice; for Chd7cKO, g-ratio = 0.9330 ± 0.003088, n = 126 remyelinated axons from 4 mutant mice; p < 0.0001, t = 6.440; Two-tailed unpaired Student’s t test). Image published in: He D et al. (2016) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

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