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Figure 4. Temperature activation involves conformational changes in the TM helices. (A) Example traces showing that temperature-dependent activation of whole-cell TREK-2 currents from 22°C (left) to 37°C (right) markedly decreases inhibition by 100 µM NFx. For comparison, the currents at 22°C are also shown in gray in the right panel. Currents were recorded by voltage protocols similar to those used in Fig. 1 D. (B) Summary dose–response curves showing reduced NFx sensitivity of whole-cell TREK-2 currents at 22°C, 37°C, and 42°C (data shown are percent inhibition of basal currents recorded at 0 mV; mean ± SEM, n ≥ 6). IC50 at 22°C, 23.4 ± 3.1 µM; but at 37°C and 42°C, >100 µM. (C) A truncated version of TREK-2 (TREK2 ΔN/ΔC) that no longer responds to temperature. Fold activation represents change in current between 22°C and 37°C. (D) The NFx sensitivity of this temperature-insensitive mutant is unaffected by temperature, indicating a specific effect on WT TREK-2. (C and D) Error bars shown are mean ± SEM (n ≥ 6). Image published in: McClenaghan C et al. (2016) © 2016 McClenaghan et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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