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Figure 4. Membrane potential responses to depolarizing current pulses in smaller and larger MNs in the dl-TMN. Aa, A brainstem slice including the dl-TMN. A stimulation electrode was placed just dorsal to the TMN. PrV, Trigeminal principle nucleus; VIIn, facial nerve; sV, sensory root of the trigeminal nerve. Scale bar, 500 μm. Ab, A simultaneous recording obtained from a pair of smaller and larger MNs that were filled with Lucifer yellow. Scale bar, 100 μm. Ac, A Lucifer yellow image of the smaller and larger MNs after fixation with paraformaldehyde (open and filled arrowheads, respectively). Scale bar, 100 μm. B, C, Membrane potential responses to depolarizing current pulses applied to the smaller and larger MNs (Bb and Cb, respectively) from the same baseline membrane potential of −86 mV brought about by negative DC current injection of −62 and −70 pA, respectively (Ba and Ca, respectively). The apparent spike threshold was lower in the smaller MN (open arrow) than in the larger MN (filled arrow). Image published in: Okamoto K et al. (2016) Copyright © 2016 Okamoto et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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