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Figure 8. 8-Br-cGMP inhibits cloned TASK3 channels. A, Superimposed TASK3 currents evoked by voltage steps ranging between −150 and +60 mV in 30 mV steps applied at a holding potential of −90 mV at pH 8.4 and 7.4. The respective current traces were obtained at the time points indicated with *1 and *2 in D. B, Superimposed TASK3 currents obtained at pH 7.4 before (blue traces) and during (red traces) the application of 8-Br-cGMP. The respective current traces were obtained at the time points indicated with *2 and *3 in D. C, Superimposed TASK3 currents obtained at pH 8.4 before (green traces) and during the application of 8-Br-cGMP (orange traces). The respective current traces were obtained at the time points indicated with *1 and *4 in D. D, Plots of TASK3 currents at 0, +30, and +60 mV against time. Gray horizontal bars represent the timing and duration of perfusion of the extracellular solutions at respective pH, and the black horizontal bar represents the timing and duration of the addition of 100 μm 8-Br-cGMP. Arrowheads indicate the time points at which the respective current responses shown in A–C were obtained. E, The normalized TASK3 currents recorded before and during the application of 8-Br-cGMP at pH 7.4 (blue and red columns, respectively) and those recorded before and during the application of 8-Br-cGMP at pH 8.4 (green and orange columns, respectively; n = 8). All of the TASK3 currents are normalized by that obtained before the application of 8-Br-cGMP at pH 8.4. #p < 0.05, two-way ANOVA followed by Fisher’s PLSD. F, The normalized TASK1 currents recorded before and during the application of 8-Br-cGMP at pH 7.4 (blue and red columns, respectively) and those recorded before and during the application of 8-Br-cGMP at pH 8.4 (green and orange columns, respectively; n = 5). All of the TASK1 currents are normalized by that obtained before the application of 8-Br-cGMP at pH 8.4. #p < 0.05, two-way ANOVA followed by Fisher’s PLSD. Image published in: Okamoto K et al. (2016) Copyright © 2016 Okamoto et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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