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Figure 2. AhR deficiency affects both multiciliated cell commitment and cilia organization.(a) Immunofluorescent staining of acetylated α-tubulin (cilia, violet) and Foxj1 (yellow) in wt and Ahr−/− mTEC cultures at 9 days of ALI. Scale bars, 20 μm. Dashed boxes indicate insets (i,ii). Scale bars, 5 μm (for insets i and ii). Data representative of two independent experiments. (b) Immunofluorescent staining of acetylated α-tubulin (cilia, violet) and Foxj1 (yellow) in Ahr+/+ and Ahr−/− mTEC cultures following 9 days of ALI. Caption of the z projection of a single multiciliated cell showing distribution of the acetylated α-tubulin staining in the cytoplasm. Scale bars, 2 μm. Data representative of two independent experiments. (c) Number of Foxj1+ cells per field in AhR-sufficient and AhR-deficient mTEC cultures at day 9 of ALI. Mean±s.e.m.; Student's t-test (unpaired, two-tailed). n=5 random fields per group, data from two independent experiments. (d) Fraction of Foxj1+ cells bearing a disorganized pattern of cilia (uneven distribution on the apical surface; intracytosolic staining of acetylated α-tubulin) in Ahr+/+ and Ahr−/− mTEC cultures after 9 days of ALI. Mean±s.e.m.; Student's t-test (unpaired, two-tailed). n=5 random fields per group, data from two independent experiments. Image published in: Villa M et al. (2016) Copyright © 2016, The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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