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FIGURE 2. Incorporation of titratable arginine analog, canavanine. A, l-lysine (Lys), l-arginine (Arg), and l-canavanine (Can). B, graphic illustrating nonsense suppression of Arg76UAG mRNA by co-injection of Can-ligated tRNA into Xenopus laevis oocytes (yellow/brown spheres). C, mean peak current amplitude (± S.E.) in response to 10 mm glutamate at oocytes injected with mRNA and tRNA ± Can (n = 8–15; *, p < 0.05; n.s. not significant, ANOVA). Inset shows example responses to 10 mm glutamate at oocytes (pH = 7) injected with indicated RNA combinations (scale bars: x, 5 s; y, 20 nA). D, example recordings of current responses to increasing concentrations of glutamate and mean ± S.E. peak current responses (n = 5–8; normalized to maximum glutamate-gated current) at Can76 GluCls (Can76). Scale bars: x, 10 s; y, 100 nA. E, left, glutamate-gated currents at oocytes expressing wild-type or Can76 GluCls continuously perfused at pH 7.0 or 5.8, as indicated (scale bars: x, 30 s; y, 100 nA). Right, mean ± S.E. peak current responses to 30 μm glutamate, normalized to maximum glutamate-gated current (I30 μm/Imax; n = 5–8; *, p < 0.05, ***, p < 0.001, Student's t test). Gray arrows illustrate inhibition of 30 μm glutamate-gated current. Can76 pH 7.0 recording in E is repeated from D. Image published in: Lynagh T et al. (2017) © 2017 by The American Society for Biochemistry and Molecular Biology, Inc. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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