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Figure 5. Immunofluorescence analysis of pSTAT3 levels in NR56 stage animals after SCI. (A) Scheme representing a coronal section of the spinal cord from an NR56 animal: Vz, ventricular zone; Mn, motor neuron columns; Gm, grey matter; Ve, vertebra; Drg, dorsal root ganglion; Nt, notochord. (B)−(E*) Coronal sections through the spinal cord, analyzed for immunostaining of pSTAT3 (green) and TOTO3 staining (nuclei, blue). (B), (B′) Section from a sham operated animal at 1 dpt. (C) (C′) Section from the spinal cord stump at 3 hpt. (D)−(E*) Sections at 3 hpt (D), (D*) and 1 dpt (E), (E*) 200 μm caudal to the lesion; boxed areas indicate a digital zoom of grey matter and motoneuron columns. Arrows indicate representative pSTAT3+ motoneurons (white arrows, cells with large nuclei) and cells in the grey matter (white arrowheads). White bars indicate 100 μm (B)−(D′), (E), (E′) and 30 μm (D′), (D*), (E′), (E*). The sections depicted are representative of two animals per time point. Image published in: Tapia VS et al. (2017) © 2017 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue stat3.L aprf, hies, stat3.1, Xstat3 X. laevis Sometime during NF stage 56 to NF stage 66 spinal cord regenerating tissue motor neuron Image source: Published Larger Image Printer Friendly View

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