Xenbase Image ID: 170277

Figure 1. Inhibition results from enzymatic activity of SMase: (a) WT-human-CFTR currents elicited with 0.1 mM IBMX and a voltage step to −60 mV were inhibited by 2 μg/mL WT SMase but not by the inactive H322A mutant (n = 6). (b) Example traces show that inhibition rate was proportional to SMase concentration. For display, currents were normalized to the amplitude 1 minute prior to application of enzyme. SMase was applied from 0–10 minutes. Sensitivity to GlyH-101 applied at the end of the experiment shows that remaining currents represented uninhibited CFTR channels. (c) Summary data showing that fractional inhibition at 10 minutes was proportional to SMase concentration (n = 4–6). Image published in: Stauffer BB et al. (2017) © The Author(s) 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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