Xenbase Image ID: 170552

Fig. S4. The expression of the 123-gene early dorsal β-catenin signature examined via principal component analysis (PCA) to analyze dimensionality in six experimental conditions: Cerberus/control (Cer/con), β-catenin morpholino/control (β-CatMO/con), Siamois/control (Sia/con), lithium chloride/control (LiCl/con), dorsal/ventral regenerating halves (Dor/Ven), and Wnt8/control (Wnt/con). Each axis represents a principal component (PC), with the first one having the most variation. PCA analysis confirmed the reproducibility of the early β-catenin signature by clustering replicate conditions together and distinguishing ventralizing (β-catenin depletion) and dorsalizing treatments. The Cerberus mRNA-injected condition was located between the β-CatMO and the rest of the sequenced conditions due to its ability to inhibit both Nodal and BMP target genes. Image published in: Ding Y et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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