Xenbase Image ID: 170554

Fig. 6. Comparison of the expression of the early dorsal β-catenin gene signature at blastula and gastrula stages. (A) Heat map showing that the majority of the early dorsal β-catenin gene signature had higher expression levels (in red) in WT control (Cont.) embryos at stage 10.5 rather than at blastula stage 9. RPKMs of the genes constituting the early β-catenin signature genes at blastula stage 9 or gastrula stage 10.5 (results from five and six sequenced libraries, respectively) were normalized by row. (B) The majority of genes in the early β-catenin signature were unaffected by β-CatMO at stage 9; the effect of β-catenin MO becomes evident at stage 10.5. (C) Heat map comparing RPKM levels of the early dorsal β-catenin signature in 46 libraries at blastula and gastrula stages. Only a minority of the β-catenin signature were inhibited by β-CatMO or activated by Wnt-like signals at blastula stage 9. RPKM values of all libraries were allowed to cluster automatically according to rows. The cluster delimited by a box at the top indicates transcripts that are capable of being induced or repressed by dorsalizing or ventralizing treatments at blastula stage 9. A list of these genes is provided in Dataset S8 and includes many known early Wnt targets, such as Siamois, Xnr3, Xnr5, Xnr6, Noggin, and ADMP. The scale bar at the bottom indicates normalized RPKM values. In conclusion, most genes in the early β-catenin signature become sensitive to β-catenin depletion only at gastrula stage, presumably indicating the requirement of the endodermal and mesodermal germ layer induction for Spemann organizer development. Cer, Cerberus; Cont, control; St., stage. Image published in: Ding Y et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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