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Figure 5. Regulation of znf703 expression by neural crest inducing signals. (a) mRNAs encoding noggin (1 ng) and wnt1 (100 pg) were injected, alone or in combination with pax3MO, into both blastomeres in the animal pole region at the 2-cell stage. At the blastula stage (NF Stage 9), animal cap explants were dissected, cultured for 8 hr at room temperature (equivalent Stage 13/14) and analyzed by qRT-PCR. (b) In combination, Noggin and Wnt1 induce snai2 and repress keratin expression. Co-injection of pax3MO blocks snai2 induction by Noggin and Wnt1 and promotes neural fate (sox2 expression). The induction of znf703 by Noggin and Wnt1 is unaffected by injection of pax3MO Image published in: Hong CS and Saint-Jeannet JP (2017) Copyright © 2017. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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