XB-IMG-170626
Xenbase Image ID: 170626
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Figure 6. XShal transcripts are detectable in young embryos by whole-mount in situ hybridization. Embryos were incubated as whole-mounts
with sense control or antisense XShal probes. The RNA probes were labeled with digoxigenin (Boehringer-Mannheim), which permitted detection
of hybridization with anti-digoxigenin antibodies coupled to alkaline phosphatase followed by reaction with the substrates NBT/BCIP to form the
purple precipitate. a, XShal hybridization signal is localized to the nervous system. Three day embryos [Nieuwkoop and Faber (NF) stage 401
hybridized either to antisense XShal probe (top) or sense control probe (bottom) were processed similarly. Note the intense staining in the head,
dorsal and ventrolateral aspects of the spinal cord. Occasionally in controls, the cement gland (arrowhead) showed background staining. However,
in the control of b, the cement gland does not show background staining. This difference may be due to the time the embryos are in substrate
solution (30 hr in a vs. 20 hr in b and c; see Materials and Methods). b and c, Slightly older embryos (NF stage 42) hybridized to antisense XShal
probes (b, right, and c, bottom) or sense control probes (b, left, and c, top) and examined at higher power demonstrate further the specificity of the
XShal signal. b, In the head, both the brain and the gill area (arrowheads) show intense staining. c, Along the length of the spinal cord, the dorsal
aspect (arrowheads) contains XShal mRNA. Scale bars, a, 700 pm; b and c, 1 mm.
Image published in: Ribera AB and Nguyen DA (1993) Copyright © 1993. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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