Xenbase Image ID: 170638

Figure 3. FN Knockdown Retards Convergence and Extension in Sandwich Explants (A) Representative 6-hr-old Keller sandwich explants from 15 μMoles FNMO-injected embryos show convergent thickening but little extension, while uninjected sandwiches and control morpholino-expressing explants (COMO) converge and extend. (B) Individual explants showing convergence of the mesoderm with respect to the ectoderm (arrowheads versus arrows) were stained for fibronectin fibrils. (C) Montages of en face maximal projections of confocal z-series of FN fibrils from the same explants shown in ([B]; dashed boxes) show that fibrils do not assemble in FNMO-expressing explants while fibrils assemble in control explants. (D) XZ-projections analogous to transverse projections of confocal z-series (shown by white boxes in [C]) show that fibrils assemble within control explants but not within the thicker sandwich explants made from FNMO-injected embryos. Arrow-ended lines indicate thickness of explant. (E) Measurements of explant thickness from five batches of sandwich explants demonstrate FNMO-expressing explants are significantly thicker than sibling control or COMO-injected explants. Image published in: Davidson LA et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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