XB-IMG-170645
Xenbase Image ID: 170645
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Fig. 2. Rarβ2 is induced by TTNPB and is regulated by RARα and/or
RARγ. (A) QPCR showing Rarα1, Rarα2, Rarβ2, Rarγ1 and Rarγ2 expression
in embryos treated at stage 7/8 with 1 μM TTNPB, 1 μM AGN193109 or vehicle
(0.1% ethanol) and collected at tailbud stage. The y-axis represents 2−δδCt
values normalized to Eef1a1 and expressed as fold induction relative to control
vehicle (n=5 biological replicates) using standard propagation of error
(Bevington and Robinson, 2003). Error bars indicate s.e.m. An unpaired t-test
in GraphPad Prism v5.0 is reported (*P≤0.05, **P≤0.01, ***P≤0.001).
(B) Embryos were injected unilaterally at the 2- or 4-cell stage with 6.6 ng Rarα
MOs or Rarγ MOs. The injected side is indicated by magenta β-gal lineage
tracer. Rarα MOs and Rarγ MOs knock down the expression of Rarβ2 (α MOs,
13/13 embryos; γ MOs, 8/8) at tailbud stage. Embryos are shown in dorsal view
with anterior on the left. Midline is indicated by a broken green line. Image published in: Janesick A et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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